Optimization and Purification of Keratinase from Bacillus anthracis with Dehairing Application

Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities.

We report the isolation of a keratinolytic-producing Bacillus subtilis strain and the characterization of the exceptional dehairing properties of its subtilisin-like keratinase. This enzyme can be an alternative to sodium sulfide, the major pollutant from tanneries, and may completely replace it. Its unique nonactivity upon collagen enhances its industrial potential.

Purification Properties of Bacillus thuringiensis TS2 Keratinase Enzyme

This study was focused on purification and characterization of keratinase from Bacillus thuringiensis TS2, isolated from feather dumping soil site. Zymogram of crude enzyme on native-PAGE presented bands with keratinase activity of molecular weights is 41 kDa. In purification steps 2.69 fold purification was achieved after 80% ammonium precipitation of the with 34.42% recovery. In further purif...

purification & separation of bacillus anthracis 34f2 antigens with uf & hplc

anthrax is one of the important zoonose disease between human and cattle. bacillus anthracis is a gram- positive bacterium which its tripartite protein toxin contains protective antigen (pa), edema factor (ef) and lethal factor (lf) and a poly d- glutamic acid capsule. a recent resurgence of interest in b.anthracis resulted in improved methods for production, separation and purification of the ...

Expression Optimization and Purification of Glutamate Endopeptidase from halo-thermo tolerant Bacillus licheniformis SL-1

Aims and Background: Glutamate-specific endopeptidase (GSE, EC 3.4.21.19) is belonging to the serine protease family enzymes. A glutamate-specific endopeptidase has the ability to cleavage peptide bonds for the protein structure analysis, solid phase synthesis of peptides and preparation of nanotubes. The purpose of this investigation was to produce glutamate endopeptidase enzyme from the Bacil...

Bacillus anthracis isolated from aborted bovine fetuses